Application

Research Sub CategoryNuclear Receptors

Anti-WAPL Antibody, clone 1C7-B1 is an antibody against WAPL for use in Western Blotting, Immunoprecipitation.

Research CategoryEpigenetics & Nuclear Function

Immunoprecipitation Analysis: 1.0 mL tissue culture supernantant of this antibody immunoprecipitated WAPL in HeLa cell lysate (Professor Haruhiko Siomi, Keio University School of Medicine).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Wings apart-like protein homolog (also known as Friend of Epstein-Barr virus nuclear protein 2) is a nuclear protein encoded by the WAPL (WAPAL) gene in human. Wapl functions as a negative regulator of cohesin–chromosome association and facilitates the progression of mitosis both during interphase and prophase. In interphase, Wapl destabilizes cohesin–chromosome interaction and thereby reduces the extent of cohesin-mediated chromosome looping. In prophase, mitotic kinases phosphorylate cohesin and its regulators. Wapl then triggers the release of phosphorylated cohesin from sister chromatids, allowing sister chromosome decatenation by topoisomerase II (Topo II). (PMID 24156811).

Immunogen

Recombinant protein corresponding to human WAPL.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected WAPL in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~175 kDa observed